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pspcas9(bb)-2a-puro (px459) v2.0  (Addgene inc)


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    Structured Review

    Addgene inc pspcas9(bb)-2a-puro (px459) v2.0
    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that <t>endogenous</t> levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.
    Pspcas9(Bb) 2a Puro (Px459) V2.0, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pspcas9(bb)-2a-puro (px459) v2.0/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    pspcas9(bb)-2a-puro (px459) v2.0 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Protein Kinase C promotes peroxisome biogenesis and peroxisome–endoplasmic reticulum interaction"

    Article Title: Protein Kinase C promotes peroxisome biogenesis and peroxisome–endoplasmic reticulum interaction

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.202505040

    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.
    Figure Legend Snippet: Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.

    Techniques Used: Confocal Microscopy, CRISPR, Incubation, Control

    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm.
    Figure Legend Snippet: Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm.

    Techniques Used: Confocal Microscopy, CRISPR, Incubation



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    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that <t>endogenous</t> levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.
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    Image Search Results


    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.

    Journal: The Journal of Cell Biology

    Article Title: Protein Kinase C promotes peroxisome biogenesis and peroxisome–endoplasmic reticulum interaction

    doi: 10.1083/jcb.202505040

    Figure Lengend Snippet: Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm. See for the separate channels of control images.

    Article Snippet: We used px459 and px330 plasmids to clone CRISPR/Cas9 constructs for gene knockout and endogenous tagging. pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (plasmid #62988; Addgene; https://n2t.net/addgene:62988 ; RRID:Addgene_62988) (Ran et al., 2013). pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (The Broad Institute, Cambridge, Massachusetts, MA, USA; plasmid #42230; Addgene; http://n2t.net/addgene:42230 ; RRID:Addgene_42230) ( ). pcDNA4-PKCZeta WT His tag was a gift from Jeff Wrana (Mount Sinai Hospital, Toronto, Canada; plasmid #24609; Addgene; https://n2t.net/addgene:24609 ; RRID:Addgene_24609) ( ).

    Techniques: Confocal Microscopy, CRISPR, Incubation, Control

    Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm.

    Journal: The Journal of Cell Biology

    Article Title: Protein Kinase C promotes peroxisome biogenesis and peroxisome–endoplasmic reticulum interaction

    doi: 10.1083/jcb.202505040

    Figure Lengend Snippet: Kinase Inhibitor Screen. Confocal microscopy of the HEK293T CRISPR/Cas9 PMP70-GFP cells incubated for 2 days with 1 μM of the indicated small molecule. Note that endogenous levels of PMP70 vary between treatments, as do the numbers of peroxisomes. Scale bar: 5 μm.

    Article Snippet: We used px459 and px330 plasmids to clone CRISPR/Cas9 constructs for gene knockout and endogenous tagging. pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (plasmid #62988; Addgene; https://n2t.net/addgene:62988 ; RRID:Addgene_62988) (Ran et al., 2013). pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (The Broad Institute, Cambridge, Massachusetts, MA, USA; plasmid #42230; Addgene; http://n2t.net/addgene:42230 ; RRID:Addgene_42230) ( ). pcDNA4-PKCZeta WT His tag was a gift from Jeff Wrana (Mount Sinai Hospital, Toronto, Canada; plasmid #24609; Addgene; https://n2t.net/addgene:24609 ; RRID:Addgene_24609) ( ).

    Techniques: Confocal Microscopy, CRISPR, Incubation